Genome Editing of Pluripotent Cells Workshop

This workshop is ideal for trainees who want to advance their biological knowledge and research projects through the use of cutting edge gene-modulating technologies. The taught techniques are valuable to both human and mouse stem cell research, modifying cells through a transgenic approach. The workshop will be 60% bench work and 40% classroom (talks and in silico analysis). By the end of the workshop trainees will be knowledgeable in designing and generating appropriate expression constructs and CRISPR/Cas9 strategies.  The course is tailored so that participants design and generate constructs for their very own research projects.

Skills and techniques taught on the course

  • Genome modification by CRISPR mediated gene editing (applicable to knock-outs and knock-ins).
  • Strategies for generating reporter cell lines. (identifying the genomic region of interest, appropriate fluorescent protein, method of genomic integration and the genomic location).
  • Human pluripotent cells culture, transfection, subcloning and genotyping.
  • Generation of a gRNA/Cas9 plasmid that you can use in your own research
  • Gene over-expression with transposons in human and mouse stem cells (selecting a promoter, a method of genomic integration and identifying amenable genomic loci).

View the workshop agenda – to come
Workshop Date: November 28-29, 2017
Registration fee: $500 (CAD)
Workshop Location: The Child and Family Research Institute, Vancouver
Application Deadline: Wednesday October 4, 2017

Download the Workshop and Bursary Application Form here

 


Application procedure and registration fees:

Space in this workshop is limited; therefore all those interested in participating must submit an application form (see below). Selected applicants will be notified by the middle of October, and will be instructed on how to proceed with registration for the workshop.

Applicants will be required to submit a resume as well as a brief project proposal outlining the specifics of their research and CRISPR project.

The registration fee for this workshop is $500 ($CAD).

The Stem Cell Network (SCN) and ThéCell are offering a limited number of travel awards to enable eligible trainees to attend this workshop. All those who submit an application to attend will automatically be considered for trainee sponsorship and travel bursaries through the appropriate networks; SCN or ThéCell.

Download the Workshop and Bursary Application Form here.

Your completed application form and supporting documents should be send to info@stemcellnetwork.ca by 11:59 PM (sender’s time) Wednesday, October 4, 2017.

For more information please contact jbijl@stemcellnetwork.ca

 


Travel support available:

This SCN/ThéCell travel award provides financial assistance for trainees to attend the Genome Editing of Pluripotent Cells Workshop, November 28-29, 2017, at the Child and Family Research Institute in Vancouver, organized by Dr. Francis Lynn.

Details of the SCN & ThéCell Travel Award:

  • The SCN/ ThéCell travel award will cover travel expenses associated with attending the workshop, for trainees not based in Vancouver:
  • Reimbursement will be processed following the workshop and in accordance with the SCN Travel Booking & Reimbursement Policy or the ThéCell Travel Policy (policies will be provided to the selected applicants).
  • Accommodation during the course will be provided for trainees not based in Vancouver.  Two trainees of the same gender may be required to share a room.

Eligibility: 

The Stem Cell Network*, and the Quebec Network in Tissue and Cell Therapy (ThéCell)‡ are offering a limited number of travel awards to enable eligible trainees to attend this workshop. All those who submit an application to attend will automatically be considered for trainee sponsorship and travel bursaries through the appropriate networks; SCN or ThéCell

* To be eligible for a SCN travel award the applicant must work in a Canadian research institute or university work on a project in stem cells or regenerative medicine. If you’re unsure whether you are a SCN HQP, please email Rebecca Cadwalader at rcadwalader@stemcellnetwork.ca for confirmation.

To be eligible for a ThéCell travel award the applicant must be a MSc Student, PhD Student or PDF work in the lab of a ThéCell principal investigator work on a project in stem cells or regenerative medicine. If you’re unsure whether you are an ThéCell trainee, please contact Friederike Pfau at fpfau@loex.qc.ca for confirmation.


Application procedure:

  1. Complete the application form and return it, along with any additional documents required, by email to info@stemcellnetwork.ca by 11:59 PM (sender’s time) Wednesday, October 4, 2017.
  2. The SCN Training & Education Committee will review all complete applications, and applicants will be informed of the competition outcome by the middle of October.